Energy Transduction by Ion Pumps

  • Functional and shunt states of bacteriorhodopsin resolved by 250 GHz dynamic nuclear polarization-enhanced solid-state NMR. Bajaj VS, Mak-Jurkauskas ML, Belenky M, Herzfeld J and Griffin RG. Proc Natl Acad Sci USA 106, in press (2009). Abstract

  • Energy transformations early in the bacteriorhodopsin photocycle revealed by DNP-enhanced solid-state NMR. Mak-Jurkauskas ML, Bajaj VS, Hornstein MK, Belenky M, Griffin RG and Herzfeld J. Proc Natl Acad Sci USA 105, 883-888 (2008). Abstract PDF

  • Solid-State NMR Investigation of the Buried X-Proline Peptide Bonds of Bacteriorhodopsin. Lansing JC, Hu JG, Belenky M, Griffin RG and Herzfeld J. Biochemistry 42, 3586-3593 (2003). Abstract PDF

  • Magnetic Resonance Studies of the Bacteriorhodopsin Pump Cycle. Herzfeld J and Lansing JC. Annual Reviews of Biophysics and Biomolecular Structure 31, 73-95 (2002). Abstract PDF

  • Tryptophan Interactions in Bacteriorhodopsin: A Heteronuclear NMR Study. Petkova, AT, Hatanaka M, Jaroniec CP, Hu JG, Belenky M, Verhoeven M, Lugtenburg J, Griffin RG and Herzfeld J. Biochemistry 41, 2429-2437 (2002). Abstract PDF

  • Control of the Pump Cycle in Bacteriorhodopsin: Evolution of the H-C14-C15-H Dihedral Angle Detected by Solid-State NMR. Hatcher ME, Hu JG, Belenky M, Verdegem P, Lugtenburg J, Griffin RG and Herzfeld J. Biophysical J 82, 1017-1029 (2002). Abstract PDF

  • Chromophore Distortions in the Bacteriorhodopsin Photocycle: Evolution of the H-C14-C15-H Dihedral Angle Detected by Solid-State NMR. Lansing JC, Hohwy M, Jaroniec CP, Creemers AFL, Lugtenburg J, Herzfeld J and Griffin RG. Biochemistry 41, 431-438 (2002). Abstract PDF

  • Measurement of Dipolar Couplings in a Uniformly 13C,15N-Labeled Membrane Protein: Distances between the Schiff Base and Aspartic Acids in the Active Site of Bacteriorhodopsin. Jaroniec CP, Lansing JC, Tounge BA, Belenky M, Herzfeld J and Griffin RG. J Am Chem Soc 123, 12929-12930 (2001). PDF

  • NMR Probes of Vectoriality in the Proton-Motive Photocycle of Bacteriorhodopsin: Evidence for an "Electrostatic Steering" Mechanism. Herzfeld J and Tounge BA. BBA Bioenergetics 1460, 95-105 (2000). Abstract PDF

  • Structural Investigation of the Active Site in Bacteriorhodopsin: Geometric Constraints on the Roles of Asp-85 and Asp-212 in the Proton-Pumping Mechanism from Solid State NMR. Griffiths JM, Bennett AE, Engelhard M, Siebert F. Raap J, Lugtenburg J, Herzfeld J and Griffin RG. Biochemistry 39, 362-371 (2000). Abstract PDF

  • Arginine Activity in the Proton-Motive Photocycle of Bacteriorhodopsin: Solid-State NMR Studies of the Wild-Type and D85N Proteins. Petkova AT, Hu, JG, Sun BQ, Bizounok M, Simpson M, Griffin RG and Herzfeld J. Biochemistry 38, 1562-1572 (1999). Abstract PDF

  • Early and Late M Intermediates in the Bacteriorhodopsin Photocycle: A Solid State NMR Study. Hu, JG, Sun BQ, Bizounok M, Hatcher ME, Lansing JC, Raap J, Verdegem PJE, Lugtenburg J, Griffin RG and Herzfeld J. Biochemistry 37, 8088-8096 (1998). Abstract PDF

  • The Pre-Discharge Chromophore in Bacteriorhodopsin: A 15N Solid State NMR Study of the L-Photointermediate. Hu JG, Sun BQ, Petkova AT, Griffin RG and Herzfeld J. Biochemistry 36, 9316-9322 (1997). Abstract PDF

  • Solid State 13C and 15N NMR Investigations of the N Intermediate of Bacteriorhodopsin. Lakshmi KV, Farrar MR, Raap J, Lugtenburg J, Griffin RG and Herzfeld J. Biochemistry 33, 8853-8857 (1994). Abstract PDF

  • Internuclear Distance Measurement in a Reaction Intermediate: Solid-State 13C NMR Rotational Resonance Determination of the Schiff Base Configuration in the M Photointermediate of Bacteriorhodopsin. Lakshmi KV, Auger M, Raap J, Lugtenburg J, Griffin RG and Herzfeld J. J Am Chem Soc 115, 8515-8516 (1993). PDF

  • Solid-State NMR Study of [e-13C]Lys-Bacteriorhodopsin: Schiff Base Photoisomerization. Farrar MR, Lakshmi KV, Smith SO, Brown RS, Raap J, Lugtenburg J, Griffin RG and Herzfeld J. Biophysical J 65, 310-315 (1993). Abstract PDF

  • Rotational Resonance NMR Study of the Active Site Structure in Bacteriorhodopsin: Conformation of the Schiff Base Linkage. Thompson LK, McDermott AE, Raap J, van der Wielen CM, Lugtenburg J, Herzfeld J and Griffin RG. Biochemistry 31, 7931-7938 (1992). Abstract PDF

  • Mechanism of Proton Pumping in Bacteriorhodopsin by Solid State NMR: The Protonation State of Tyrosine in the Light Adapted and M States. McDermott AE, Thompson LK, Winkel C, Farrar MR, Pelletier S, Lugtenburg J, Herzfeld J and Griffin RG. Biochemistry 30, 8366-8371 (1991). Abstract PDF

  • Solid State 13C NMR Study of Tyrosine Protonation in Dark-Adapted Bacteriorhodopsin. Herzfeld J, Das Gupta SK, Farrar MR, Harbison GS, McDermott A, Pelletier SL, Raleigh DP, Smith SO, Winkel C, Lugtenburg J and Griffin RG. Biochemistry 29, 5567-5574 (1990). Abstract PDF

  • Solid-State NMR Detection of Proton Exchange Between the Bacteriorhodopsin Schiff Base and Bulk Water. Harbison GS, Roberts JE, Herzfeld J and Griffin RG. J Am Chem Soc 110, 7221-7223 (1988).PDF